CryoBank - How to Use
 1. From
a fresh 18 hours plate culture of bacteria, make a heavy,
cloudy suspension of the organism in the CRYOBANK™
medium in the tube. |
 2. Mix
the tubes by shaking and inverting the tube to allow
the bacteria in the suspension to coat the beads |
 3. Using
a sterile pipet remove the CRYOBANK™ medium from
the tube. |
 4. Immediately
place the CRYOBANK™ tube in a -70°C freezer
or under liquid nitrogen to store the culture. |
RECOVERY
 1. Remove
the CRYOBANK™ tube from the freezer or liquid nitrogen
container. Remove the cap of the CRYOBANK™ tube.
Using forceps or a inoculating needle remove one bead. |
 2. Roll
the bead over the surface of appropriate plate culture
medium or drop the bead into a nutrient broth. Immediately return the CRYOBANK™ tube with remaining
beads to the freezer or liquid nitrogen storage container
to prevent other beads from thawing. |